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high molecular weight dna中文是什么意思

  • 高分子质量dna

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  • In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ) , and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee . the plasmid pfb 1 - cee was transformed into dh1 obac competent e . coli cells . high molecular weight dna was prepared from the overnight cultures from the selected e . coli colonies , which was recombinant baculovirus shuttle vector containing hcv structural gene , named bac - cee
    本实验用pcr扩增hcv结构区基因,克隆到杆状病毒表达载体pfastbacl中,构建成重组转座载体pfb1 - cee ,转化dh10bac大肠杆菌感受态细胞,筛选阳性菌落,抽提大分子质粒dna ,获得含hcv结构区基因的重组杆状病毒穿梭载体bac - cee ,脂质体介导转染sf9昆虫细胞,出现细胞病变后,收集含有重组杆状病毒颗粒的培养上消,重新感染sf9细胞,收集sf9细胞,进行12 . 5 sds -聚丙烯酰胺凝胶电泳,可见表达的蛋白条带。
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